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Researching and Writing

Publications (2019 onwards)

  1. Suhasini Mallick, Moupriya Nag, Dibyajit Lahiri,Soumya Pandit,Tanmay Sarkar, Siddhartha Pati, Nilesh Prakash Nirmal, Hisham Atan Edinur, Zulhisyam Abdul Kari, Muhammad Rajaei Ahmad Mohd Zain,Rina Rani Ray Engineered Nanotechnology: An Effective Therapeutic Platform for the Chronic Cutaneous Wound Nanomaterials 2022, 12(5), 778; https://doi.org/10.3390/nano12050778 

  2. Dibyajit Lahiri, Moupriya Nag, Bandita Dutta, Ankita Dey, Tanmay Sarkar, Siddhartha Pati, Hisham Atan Edinur, Zulhisyam Abdul Kari, Noor Haslina Mohd Noor, Rina Rani Ray Bacterial Cellulose: Production, Characterization and Application as Antimicrobial Agent Int. J. Mol. Sci. 2021, 22(23),12984; https://doi.org/10.3390/ijms222312984

  3. S. Ghosh , D Lahiri, , M Nag, , A Dey , S Pandit , T Sarkar , S Pati , Z Abdul Kari , A Razali Ishak, H. A Edinur and R. R. Ray Phytocompound Mediated Blockage of Quorum Sensing Cascade in ESKAPE Pathogens Antibiotics 2022, 11, 61. https://doi.org/10.3390/antibiotics11010061

  4. Sreejita Ghosh · Dibyajit Lahiri · Moupriya Nag · Ankita Dey · Tanmay Sarkar·Riya Biswas · Bandita Dutta · Dipro Mukherjee · Siddhartha Pati ·Smaranika Pattanaik · Rina Rani Ray  Analysis of Antibiofilm Activities of Bioactive Compounds from Honeyweed (Leonurus sibiricus) Against P. aeruginosa: an In Vitro and In Silico Approach. Applied Biochemistry and Biotechnology 2021 https://doi.org/10.1007/s12010-021-03797-1

  5. M Nag, D Lahiri, A. Dey, T. Sarka, S Joshi and R. R. Ray. Evaluation of algal active compounds as potent antibiofilm agent. J. Basic Microbiol. 1–12, 2021. DOI: 10.1002/jobm.202100470

  6. Gupta, S. Banerjee, S. Pandit, P. K. Gupta, A. S.Mathriya, S. Kumar, D. Lahiri, M. Nag, R. R. Ray, S. Joshi, A comprehensive review on enhanced production of microbial lipids for high-value applications, Biomass Conversion and Biorefinery, https://doi.org/10.1007/s13399-021-02008-5

  7. Patwardhan SB, Savla N, Pandit S, Gupta PK, Mathuriya AS, Lahiri D, Jadhav DA, Rai AK, KanuPriya, Ray RR, Singh V, Kumar V, Prasad R. Microbial Fuel Cell United with Other Existing Technologies for Enhanced Power Generation and Efficient Wastewater Treatment. Applied Sciences. 2021; 11(22):10777. https://doi.org/10.3390/app112210777

  8. Marzuqa Quraishi, Kayinath Wani, Soumya Pandit, Piyush Kumar Gupta, Ashutosh Kumar Rai, Dibyajit Lahiri ,Dipak A. Jadhav, Rina Rani Ray, Sokhee P. Jung, Vijay Kumar Thakur, Ram Prasad, Valorisation of CO2 into Value-Added Products via Microbial Electrosynthesis (MES) and Electro-Fermentation Technology Fermentation 2021, 7(4), 291; https://doi.org/10.3390/fermentation7040291

  9. D. Lahiri, M. Nag; A. Dey; T. Sarkar; S. Pattnaik; S. Ghosh; H. A. Edinur; S. Pati; Z. A.Kari; R. R. Ray, Exosome Associated Host-Pathogen Interaction: A Potential effect of Biofilm Formation, Journal of Analytical Science and Technology, 2021

  10. D. Lahiri , M. Nag, A. Dey, T. Sarkar, S. Joshi, S. Pandit, A. P. Das, S. Pati, S. Pattanaik , R. R. Ray, Biofilm Mediated                 Degradation                            of  Petroleum            Products, Geomicrobiology Journal,                        2021, https://doi.org/10.1080/01490451.2021.1968979

  11.   D. Lahiri, M. Nag, S. Garai , D. Mukherjee, R R Ray ,Regulation of β-Amylase Synthesis: A Brief Overview, Molecular Biology Reports, 2021, https://doi.org/10.1007/s11033-021-06613-5

  12. M. Nag, D. Lahiri, D. Mukherjee, R. Banerjee, S. Garai, T. Sarkar, S. Ghosh, A. Dey, S. Ghosh, S. Pattanaik, H. A. Edinur, Z.A. Kari, S. Pati , R R Ray, Functionalized Chitosan Nanomaterials: A Jammer to Quorum Sensing, Polymers (MDPI) 2021 13(15),2533; https://doi.org/10.3390/polym13152533

  13. A. Jasu, R R Ray, Biofilm mediated strategies to mitigate heavy metal pollution: A critical review in metal bioremediation, Biocatalysis and Agricultural Biotechnology,2021, 37, 102-183, https://doi.org/10.1016/j.bcab.2021.102183.

  14. S.Ghosh, A. Jasu and R. R Ray Hexavalent chromium bioremediation with insight into molecular aspect: an overview Bioremediation Journal. https://doi.org/10.1080/10889868.2021.1884529

  15. D. Lahiri, S. Chakraborti, A. Jasu, M. Nag, B. Dutta, S. Dash, R R Ray, Production and purification of bacteriocin from Leuconostoc lactis SM 2 strain, Biocatalysis and Agricultural Biotechnology, 2020, 30,101845

  16. D. Lahiri, M. Nag, H. I. Sheikh, T. Sarkar, H. Edinur, S. Pati, R R Ray, Microbiologically synthesized nanoparticles and their role in silencing the biofilm signaling cascade, Frontiers in Microbiology, doi: 10.3389/fmicb 2021.636588, 2021

  17. D. Lahiri, M. Nag, I. Mukherjee, S. Ghosh, A. Dey, R R Ray.Catechin as the most efficient bioactive compound from Azadirachta indica with antibiofilm and antiquorum sensing activities against dental biofilm: an in vitro and in silico study. Applied Biochemistry and Biotechnology, 2021 26. doi:10.1007/s12010-021-03511- 1

  18. D. Lahiri, M. Nag, T. Sarkar, B. Dutta, R R Ray. Antibiofilm activity of α-Amylase from Bacillus subtilis and prediction of the optimized conditions for biofilm removal by Response Surface Methodology (RSM) and Artificial Neural Network (ANN). Applied Biochemistry and Biotechnology, Springer, 2021 Mar 1. doi: 10.1007/s12010-021-03509-9

  19. Paul, O., Jasu, A., Lahiri D., Nag M., Ray R.R., Rina Rani In situ and Ex situ Bioremediation of Heavy Metals: The Present Scenario, Journal of Environmental Engineering and Landscape Management 2021 (Accepted for Publication 21-Dec-2020)

  20. S. Ghosh, D. Lahiri, M. Nag, A. Dey T. Sarkar, S K Pathak, H A Edinur, S. Pati, R R Ray, Bacterial biopolymer: Its role in pathogenesis to effective biomaterials, Polymers (MDPI), 13(8), 2021.

  21. M. Nag, D. Lahiri, B. Dutta, G. Jadav, R. R.Ray Biodegradation of used polyethylene bags by a new marine strain of Alcaligenes faecalis LNDR-1, Environmental Science and Pollution Research, DOI : 10.1007/s11356-021- 13704-0,(Accepted for Publication 29-Mar-2021)

  22. D.Lahiri, M. Nag, R. Banerjee, D. Mukherjee, S. Garai, H. Sheikh, T. Sarkar, H. A. Edinur, S. Pati, R. R. Ray, Amylases: biofilm inducer or biofilm inhibitor? Frontiers Cellular and Infection Microbiology 2021(DOI: 10.3389/fcimb.2021.660048)

  23. M. Nag, D. Lahiri, S. Dash, S. Garai, D. Mukherjee, R. Banerjee, R R Ray, Strategies of Nanomaterial Application for Enhanced Wound Curing: An Overview, Nano Biomed. Eng., 13 (2), 2021

  24. M. Nag, D. Lahiri, S. Pati, T. Sarkar, S. Ghosh, A. Dey, H. A.Edinur, R R Ray, Microbial fabrication of Nanomaterial & its role in Disintegration of Exopolymeric matrices of Biofilm, Frontiers in Chemistry, 2021 doi: 10.3389/fchem.2021.690590

  25. D. Lahiri, M. Nag, D. Mukherjee, S.Garai, R. Banerjee, R R Ray, Recent trends in approaches for optimization of process parameters for the production of microbial cellulase from wastes, Environmental Sustainability (2021).

  26. D. Lahiri, M. Nag, B. Dutta, S. Dey, D. Mukherjee, S. J. Joshi, R. R.Ray, Antibiofilm and Anti-Quorum sensing Activities of Eugenol and Linalool From Ocimum tenuiflorum against Pseudomonas aeruginosa Biofilm, Journal of Applied Microbiology, 2021

  27. D. Lahiri, M. Nag, B. Dutta, T.Sarkar, R R Ray. Artificial Neural Network and Response Surface Methodology- Mediated Optimization of Bacteriocin Production by Rhizobium leguminosarum. Iran J Sci Technol Trans Sci 2021.

  28. B.Ghosh, D.Lahiri, M.Nag, S.Dash, R.R.Ray. Bio characterization of purified isoamylase from Rhizopus oryzae, Preparative Biochemistry And Biotechnology,50: 453-459, 2020, doi: 10.1080/10826068.2019.1706561.

  29. B.   Dutta,   D.   Lahiri,   M.   Nag,   R.R.Ray.         Bacterial Biofilm   and   Biofilm   associated   environmental protection,American Journal of applied biotechnology research, 1:1-15,2020

  30. D. Lahiri, I. Mukherjee, S. Ghosh, S. Biswas, M. Nag, R.R.Ray Green-synthesized Silver Nanoparticle as Effective Antibiofilm Agent, American Journal of applied biotechnology research, 8-21, 2019

  31. D. Lahiri, M. Nag ,Garai S. ,R.R.Ray. The Chemistry of Antibiofilm Phytocompounds, Mini Reviews in Medicinal Chemistry, 2021, doi: 10.2174/1389557520666200807135243.

  32. D. Lahiri, M. Nag, S. Dey , B. Dutta , S. Dash,R.R.Ray. (2020) Phytocompounds of Curcuma longa extract are more effective against bacterial biofilm than pure curcumin only: An in-vitro and in-silico analysis, Kuwait Journal of Sciences, 48:2

  33. D. Lahiri, M. Nag, S. Dash, B. Dutta, R.R.Ray (2020) Studies on blocking of replicase of human corona virus by bioactive compounds-In silico and Molecular Docking Studies, International Journal of Scientific Research in Biological Sciences,7 (3):19-24

Biofilm-Mediated Diseases: Causes and Controls. Springer, Singapore. 2021
Ray R.R., Nag M., Lahiri D. (eds)

The book “Biofilm-Mediated Diseases: Causes and Controls” aims to combine different communities to effectively advance the knowledge of microbial domains and specifically explore the crossing point between these disciplines. The chapters first describe sequentially the composition of biofilm, the quorum sensing, and signaling mechanism behind its formation, normal microbiota, and commensals in biofilm in various parts of our body. Various deadly diseases concerned with human natural microbiota are discussed. Next, the biofilm-associated acute and chronic diseases will be elaborately discussed with special reference to nosocomial diseases, the root cause of high percentage of morbidity, and mortality worldwide. Since the failure of host immune system indirectly accelerates the persistence of the biofilmassociated pathogenic cells and their gradual manifestations as chronic diseases, the changed scenario of immune response in the presence of biofilm is discussed with a hint that sometimes the biofilm-associated cells can change the action of immune response even in favor of biofilm formation. As the chance of postoperative safekeeping of the patient is severely hampered by the profuse growth of biofilm on implants, prosthetics, and medical appliances, it needs to be illustratively discussed. The cardinal feature of these pathogens is their resistance to antimicrobials; hence, before designing a befitting drug, the possible mechanism of antimicrobial resistance offered by the particular pathogen, including its genetic basis is required to be found out. The last part of the book deals with the control and obviously included the inhibition of quorum sensing pathway and discussion on new strategies for the treatment of biofilm-mediated diseases that includes application of phytomedicine, nanomedicine, anti-QS enzyme treatment, phage therapy, and so on. Finally, a case report dealing with the isolation and identification of a biofilm forming bacteria from central venous catheter-associated bacteria from a patient is recorded, which confirms the severity of the biofilm in production of various diseases. This book will be potentially useful for both clinicians and researchers who are dealing with infections associated with biofilms. This book will attempt to compile the different information available on recent advancements on various functional aspects of microbial biofilms, its pathogenesis, and present-day treatment strategies. Finally, the book will also elucidate a comprehensive yet a representative description of a large number of challenges associated with bacterial biofilm, viz., virulence, pathogenesis, antibiotic resistance, cell signaling, immune evasion, and is also a take home for researchers to develop effective strategies to combat these threatening diseases.

​Phytocompound Mediated Blockage of Quorum Sensing Cascade in ESKAPE Pathogens

Increased resistance of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp. (ESKAPE) pathogens against various drugs has enhanced the urge for the development of alternate therapeutics. Quorum sensing (QS) is a density dependent cell-to-cell communication mechanism responsible for controlling pathogenicity with the regulation of gene expression. Thus, QS is considered a potential target for the development of newer anti-biofilm agents that do not depend on the utilization of antibiotics. Compounds with anti-QS effects are known as QS inhibitors (QSIs), and they can inhibit the QS mechanism that forms the major form in the development of bacterial pathogenesis. A diverse array of natural compounds provides a plethora of anti-QS effects. Over recent years, these natural compounds have gained importance as new strategies for combating the ESKAPE pathogens and inhibiting the genes involved in QS. Different pharmacognostical and pharmacological studies have been carried out so far for identification of novel drugs or for the discovery of their unique structures that may help in developing more effective anti-biofilm therapies. The main objective of this review is to discuss the various natural compounds, so far identified and their employed mechanisms in hindering the genes responsible for QS leading to bacterial pathogenesis.

Biodegradation of used polyethylene bags by a new marine strain of Alcaligenes faecalis LNDR-1

Disposable plastic bags of two different chemical compositions and colors were remediated by the application of novel mesophilic group of bacteria isolated from the banks of sea water, using a 10 week soil burial method. The new strain, LNDR-1, was identified as Alcaligens faecalis by its morphological features and 16S rRNA sequencing. LNDR-1 was able to produce extracellular enzymes such as lipase, CMCase, xylanase, and protease, having PET surface degrading activity. It was found that LNDR-1 had a better decay rate of 15.25 ± 1% and 21.72 ± 2.1% for black and white plastic bags respectively in 10 weeks without prior oxidation as compared to S. marcescens. Polyethylene degradation was confirmed by substantial weight loss, alterations in surface topology, and hydrophobicity index and was found to be directly proportional to the ability to form biofilm on the plastic surface. FTIR results suggest presence of different metabolites in the bags treated with bacterial biofilm in comparison to the control setup inferring various types of metabolic pathways. Present study also reveals the ability of the strain to utilize the used polyethylene bag as the carbon source, without any prior treatment, and as per the literature survey, the working strain is with the capacity to biodegrade plastic at a considerably appreciable rate. This study suggests effectual method for the mechanism of biodegradation of plastic mediated by extracellular enzymes and formation of biofilm.

​Production and purification of bacteriocin from Leuconostoc lactis SM 2 strain

Food bio-preservation can be accomplished by antimicrobial peptides, the bacteriocins. In the present study, bacteriocin producing bacteria were screened and isolated from various food sources. Amongst them, Leuconostoc lactis SM2 was found to produce bacteriocin, with maximum antimicrobial activity against target bacterial strains. The bacteriocin was produced in shake flasks for 24 h at pH 7.0 and temperature 37 °C. The bacteriocin was purified through subsequent steps of salting out and size exclusion chromatography thus achieving a purity of 34.75%. Elution spectrum of HPLC revealed the leucocin nature of the bacteriocin. Although leucocin showed antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas putida, Klebsiella and Serratia sp, but the highest towards E. coli upon Lactobacillus MRS agar medium. This affinity was confirmed by molecular docking studies, indicating greater binding energy towards E.coli (−123.22 kcal/mol) than S. aureus (−64.63 kcal/mol). The antimicrobial effect was most prominent if the pure leucocin was added at the lag phase of growth of the target organism. Failure of revival by the bacteriocin treated second generation cells revealed the bactericidal, not bacteriostatic nature of the protein. Sustained skin texture, freshness of seeds with characteristic sharp smell in bacteriocin treated chillies indicated the applicability of leucocin for food preservation.

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